IRGM抗体—艾普蒂

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     Gel: 10%SDS-PAGE, Lysate: 40 μg, Lane: Human hepatocellular carcinoma tissue, Primary antibody: P08447(IRGM Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 minutes    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human colon cancer tissue using P08447(IRGM Antibody) at dilution 1/60. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using P08447(IRGM Antibody) at dilution 1/60. (Original magnification: ×200)    

  
  

IRGMs (Immunity-Related GTPase Family M proteins) are a group of interferon-inducible GTPases that play critical roles in cell-autonomous immunity, particularly in defense against intracellular pathogens. In humans, the IRGM gene is a key regulator of autophagy, a cellular process that eliminates invading microbes and maintains cellular homeostasis. IRGM facilitates autophagy by coordinating the assembly of autophagy machinery and directing the destruction of pathogens like Mycobacterium tuberculosis. Dysregulation of IRGM has been implicated in autoimmune diseases (e.g., Crohn’s disease), infectious diseases, and cancer.

IRGM antibodies are tools developed to detect and study the expression, localization, and function of IRGM proteins in experimental settings. These antibodies are widely used in techniques such as Western blotting, immunofluorescence, and immunohistochemistry to investigate IRGM's role in immune responses and disease mechanisms. However, research on IRGM has faced challenges due to its low protein abundance and sequence homology with other GTPases, necessitating rigorous validation of antibody specificity. Some studies report conflicting results regarding IRGM’s subcellular localization or expression patterns, partly attributed to variability in antibody performance across different clones or experimental conditions. Despite these limitations, IRGM antibodies remain essential for advancing our understanding of autophagy-related immunity and developing therapeutic strategies targeting IRGM pathways.