NDUFB5抗体—艾普蒂

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     The image is immunohistochemistry of paraffin-embedded Human brain tissue using P09036(NDUFB5 Antibody) at dilution 1/30. (Original magnification: ×200)    

  
  

The NDUFB5 antibody is a tool used to detect the NADH:ubiquinone oxidoreductase subunit B5 (NDUFB5), a key component of mitochondrial Complex I in the electron transport chain (ETC). Complex I catalyzes NADH oxidation and ubiquinone reduction, driving proton translocation to support ATP synthesis. NDUFB5 is a nuclear-encoded, accessory subunit located in the membrane arm of Complex I, contributing to its structural integrity and enzymatic function.

Antibodies targeting NDUFB5 are essential for studying mitochondrial disorders, neurodegenerative diseases (e.g., Parkinson’s), and metabolic syndromes linked to Complex I dysfunction. Researchers use these antibodies in techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess NDUFB5 expression, localization, and alterations in disease models or genetic knockouts.

Commercial NDUFB5 antibodies are typically raised in rabbits or mice using synthetic peptides or recombinant protein immunogens. Specificity is validated via knockdown/knockout controls or siRNA experiments to confirm signal loss. Cross-reactivity with homologous proteins across species (human, mouse, rat) is common due to high sequence conservation. Proper validation ensures reliability in distinguishing NDUFB5 from other Complex I subunits (e.g., NDUFS3. NDUFV1).

Studies utilizing NDUFB5 antibodies have advanced understanding of mitochondrial bioenergetics, oxidative stress responses, and mechanisms underlying pathologies like Leigh syndrome or cancer metabolism.