PPP1CB抗体;PPP1CB Antibody—艾普蒂

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     Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    

  
  

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     Western blot analysis using PPP1CB mouse mAb against Jurkat (1), A431 (2), Hela (3), HepG2 (4), HEK293 (5), MCF-7 (6) cell lysate.    

  
  

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     Flow cytometric analysis of Jurkat cells using PPP1CB mouse mAb (green) and negative control (red).    

  
  

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     Immunohistochemical analysis of paraffin-embedded human liver tissues using PPP1CB mouse mAb with DAB staining.    

  
  

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     Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using PPP1CB mouse mAb with DAB staining.    

  
  

The PPP1CB antibody is a crucial tool in studying the function and regulation of protein phosphatase 1 catalytic subunit beta (PPP1CB), a serine/threonine phosphatase involved in diverse cellular processes. PPP1CB, a member of the PP1 family, plays a role in dephosphorylating target proteins to regulate pathways such as cell cycle progression, signal transduction, glycogen metabolism, and neuronal signaling. It forms holoenzymes by interacting with regulatory subunits, enabling substrate specificity and localization.

The PPP1CB antibody is widely used in research to detect and quantify PPP1CB expression in tissues or cells via techniques like Western blotting, immunohistochemistry, and immunofluorescence. It aids in investigating PPP1CB's involvement in diseases, including cancer (e.g., modulating oncogenic signaling), neurodegenerative disorders (e.g., tau phosphorylation in Alzheimer’s), and metabolic syndromes. Researchers also utilize it to study PPP1CB's interaction partners and post-translational modifications, such as phosphorylation, that regulate its activity.

Most PPP1CB antibodies are raised against specific epitopes, often in the C-terminal region, and validated for specificity using knockout cell lines or siRNA-mediated knockdown. Commercial antibodies vary by host species (e.g., rabbit, mouse), clonality (monoclonal/polyclonal), and conjugations (e.g., HRP, fluorescent tags). Proper controls are essential to avoid cross-reactivity with homologous subunits like PPP1CA or PPP1CC. Its applications span basic molecular biology, drug discovery, and clinical research, emphasizing its role in elucidating phosphatase-mediated regulatory mechanisms.