WDFY1抗体—艾普蒂

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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-2: Human heart tissue and Mouse heart tissue lysates, Primary antibody: P09275(WDFY1 Antibody) at dilution 1/650, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using P09275(WDFY1 Antibody) at dilution 1/35. (Original magnification: ×200)    

  
  

The WDFY1 (WD Repeat and FYVE Domain-Containing Protein 1) antibody is a tool used to study the function and localization of the WDFY1 protein, which plays roles in intracellular trafficking, autophagy, and signaling pathways. WDFY1 contains WD40 repeats involved in protein-protein interactions and a FYVE domain that binds phosphatidylinositol 3-phosphate (PI3P), linking it to endosomal and autophagosomal membranes. It has been implicated in neurodevelopment, immune regulation, and cancer progression, though its precise mechanisms remain under investigation.

Antibodies targeting WDFY1 are typically developed in rabbits or mice using immunogenic peptides or recombinant protein fragments. These antibodies are validated for applications such as Western blotting, immunofluorescence, and immunoprecipitation, often with specificity confirmed via knockout/knockdown controls. Commercial antibodies may vary in epitope recognition, requiring users to verify compatibility with their experimental systems.

Research utilizing WDFY1 antibodies has helped elucidate its interaction with autophagy-related proteins (e.g., ATG16L1) and its potential role in selective autophagy processes. Challenges include low endogenous protein expression in some tissues and cross-reactivity risks due to conserved WD40/FYVE domains. Ongoing studies aim to clarify WDFY1's involvement in diseases, including neurodegenerative disorders and cancer, making its antibody a critical reagent for mechanistic explorations.