MT-CO2抗体—艾普蒂

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     Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane 1-4: K562, 293T, 231 and A549 cell lysates, Primary antibody: P10630(MT-CO2 Antibody) at dilution 1/800, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human breast cancer tissue using P10630(MT-CO2 Antibody) at dilution 1/50. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using P10630(MT-CO2 Antibody) at dilution 1/50. (Original magnification: ×200)    

  
  

The MT-CO2 (Mitochondrially Encoded Cytochrome C Oxidase II) antibody is a crucial tool for studying the second subunit of cytochrome c oxidase (COX), a key component of the mitochondrial electron transport chain (Complex IV). MT-CO2 is encoded by mitochondrial DNA and plays a vital role in catalyzing the transfer of electrons from cytochrome c to oxygen, a process essential for ATP production. Antibodies targeting MT-CO2 are widely used to investigate mitochondrial function, oxidative phosphorylation defects, and diseases linked to mitochondrial dysfunction, such as neurodegenerative disorders, metabolic syndromes, and cancer.

These antibodies enable the detection of MT-CO2 protein levels in tissues or cells via techniques like Western blot, immunohistochemistry, or immunofluorescence. Researchers utilize them to assess COX activity alterations in conditions like Leigh syndrome, Alzheimer’s disease, or ischemic injuries, where mitochondrial impairment is a hallmark. Additionally, MT-CO2 antibodies aid in distinguishing between nuclear DNA- and mitochondrial DNA-encoded protein contributions in cellular studies.

Commercial MT-CO2 antibodies are typically validated for specificity across species, including human, mouse, and rat samples. However, variability in antibody performance may arise due to differences in mitochondrial protein isolation methods or tissue fixation protocols. Proper controls, such as COX activity assays or mitochondrial depletion experiments, are recommended to confirm results. Overall, MT-CO2 antibodies serve as indispensable tools for unraveling mitochondrial biology and its implications in health and disease.