ENPEP抗体;ENPEP Antibody—艾普蒂

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     Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    

  
  

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     Western blot analysis using XRCC6 mouse mAb against Hela (1), PC-2 (2), A549 (3), A431 (4), HepG2 (5), K562 (6) cell lysate.    

  
  

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     Immunofluorescence analysis of MCF-7 cells using XRCC6 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    

  
  

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     Flow cytometric analysis of A431 cells using XRCC6 mouse mAb (green) and negative control (red).    

  
  

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     Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissues using XRCC6 mouse mAb with DAB staining.    

  
  

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     Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using XRCC6 mouse mAb with DAB staining.    

  
  

ENPEP (glutamyl aminopeptidase), also known as CD249 or aminopeptidase A, is a zinc-dependent metalloprotease involved in regulating bioactive peptides, particularly angiotensin II and III, which play critical roles in blood pressure regulation and vascular remodeling. It is a type II transmembrane glycoprotein expressed on endothelial cells, smooth muscle cells, and certain immune and cancer cells. ENPEP’s enzymatic activity contributes to peptide hormone processing, cell adhesion, and angiogenesis, linking it to cardiovascular diseases, inflammatory disorders, and tumor progression.

Antibodies targeting ENPEP have emerged as valuable tools for studying its biological functions and pathological roles. They are widely used in research applications such as Western blotting, immunohistochemistry, and flow cytometry to detect ENPEP expression patterns in tissues or cell lines. In cancer biology, ENPEP overexpression has been observed in renal cell carcinoma, glioblastoma, and other malignancies, making it a potential therapeutic target or biomarker. Anti-ENPEP antibodies enable investigations into its role in tumor angiogenesis, metastasis, and immune evasion.

Additionally, these antibodies support translational research, including the development of ENPEP-targeted therapies like inhibitory monoclonal antibodies or enzyme-blocking drugs. Their specificity and reliability are crucial for validating experimental models and clinical samples. Despite progress, challenges remain in optimizing antibody selectivity and understanding isoform-specific functions. Overall, ENPEP antibodies bridge basic research and clinical applications, offering insights into disease mechanisms and therapeutic opportunities.