HistoneH3(monomethylK18)抗体;HistoneH3(monomethylK18) Antibody—艾普蒂

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     Western blot analysis of Histone H3 (mono methyl K18) expression in HeLa cell lysate.    

  
  

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     All lanes use the Antibody at 1:2k dilution for 1 hour at room temperature.    

  
  

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     Immunohistochemical analysis of paraffin-embedded human liver, using Histone H3 (mono methyl K18) Antibody.    

  
  

The histone H3 monomethylated at lysine 18 (H3K18me1) antibody is a critical tool for studying epigenetic regulation linked to gene expression and chromatin dynamics. Histone H3. a core component of nucleosomes, undergoes post-translational modifications (PTMs) such as methylation, which influence DNA accessibility and transcriptional activity. Monomethylation at lysine 18 (K18) is a conserved mark associated with both active and repressive chromatin states, depending on cellular context. H3K18me1 has been implicated in regulating developmental genes, maintaining stem cell pluripotency, and modulating DNA damage response pathways. Dysregulation of this mark is observed in cancers, where altered H3K18me1 levels correlate with tumor progression and patient prognosis.

This antibody specifically recognizes the monomethylated form of H3K18. distinguishing it from unmethylated, dimethylated, or trimethylated states. It is commonly used in techniques like Western blotting, chromatin immunoprecipitation (ChIP), and immunofluorescence to map H3K18me1 distribution across genomes or assess its abundance in cellular models. Researchers validate its specificity using knockout controls or competitive peptides. Studies employing this antibody have shed light on how H3K18me1 interacts with chromatin modifiers (e.g., SETD6 methyltransferase) and readers to orchestrate transcriptional programs. Its application continues to advance understanding of epigenetic mechanisms in development, disease, and therapeutic targeting.