AndrogenReceptor抗体;AndrogenReceptor Antibody—艾普蒂

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     Western blot analysis of Androgen Receptor expression in 22Rv1 cell lysate.    

  
  

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     Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, using Androgen Receptor(AR-V7 specific) Antibody.    

  
  

The androgen receptor (AR) is a nuclear hormone receptor that plays a critical role in mediating the biological effects of androgens, such as testosterone and dihydrotestosterone. It regulates gene expression by binding to specific DNA sequences, influencing cellular processes like proliferation, differentiation, and survival. AR signaling is essential for male sexual development and reproductive function but is also implicated in pathologies, including prostate cancer and androgen insensitivity syndromes.

AR antibodies are essential tools for studying the receptor's expression, localization, and function in research and diagnostics. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence to detect AR protein levels in tissues or cell lines. In prostate cancer research, AR antibody staining helps assess tumor aggressiveness, treatment response, and resistance mechanisms, as AR overexpression or mutations often drive disease progression.

These antibodies target specific AR domains, such as the N-terminal transactivation domain, DNA-binding domain (DBD), or ligand-binding domain (LBD). Monoclonal antibodies (e.g., AR441. AR-V7 specific clones) offer high specificity, while polyclonal antibodies may detect broader epitopes. Validation of AR antibodies is crucial, as post-translational modifications (e.g., phosphorylation) or splice variants (e.g., AR-V7) can affect detection. Commercial AR antibodies are routinely validated using knockout cell lines or competitive assays to ensure reliability in experimental and clinical settings.