Cytokeratin14抗体;Cytokeratin14 Antibody—艾普蒂 新品

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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: Rat brain tissue lysate, Primary antibody: P00303(GJA1 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes    

  
  

Cytokeratin 14 (CK14) is a member of the keratin family, intermediate filament proteins critical for maintaining structural integrity in epithelial cells. As a type I keratin, CK14 pairs with type II keratin K5 to form heterodimers, predominantly expressed in basal epithelial cells of stratified epithelia, such as skin, mammary glands, and prostate. This expression pattern makes CK14 a key marker for identifying basal or progenitor cell populations in tissues.

CK14 antibodies are widely used in research and diagnostics to study epithelial differentiation, tissue development, and disease. In cancer biology, CK14 expression is associated with aggressive basal-like subtypes in breast cancer and squamous cell carcinomas, aiding in tumor classification. It also serves as a tool to investigate epithelial-mesenchymal transition (EMT) and cancer stem cells.

In dermatopathology, CK14 antibodies help diagnose blistering disorders (e.g., epidermolysis bullosa simplex) caused by CK14 mutations. Additionally, they are employed in wound healing studies to track keratinocyte migration. Common applications include immunohistochemistry, immunofluorescence, and Western blotting.

Commercial CK14 antibodies are typically monoclonal, raised against specific epitopes (e.g., C-terminal regions), with validated reactivity across human and murine samples. However, interpretation requires context, as CK14 may be downregulated during epithelial differentiation or aberrantly expressed in certain malignancies. Its role in both normal homeostasis and disease underscores its versatility as a biological tool.