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  • MBD1抗体;MBD1 Antibody—艾普蒂
  • MBD1抗体;MBD1 Antibody—艾普蒂
  • MBD1抗体;MBD1 Antibody—艾普蒂

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MBD1抗体;MBD1 Antibody—艾普蒂

Rabbit Monoclonal MBD1 Antibody
询价 20μl 起订
50μl 起订
100μl 起订
上海 更新日期：2025-05-29

上海切尔齐生物科技有限公司

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联系人：周***
电话：027***
手机：180***
邮箱：nuo***

产品详情:

中文名称：
MBD1抗体
英文名称：
Rabbit Monoclonal MBD1 Antibody
品牌：
艾普蒂
产地：
武汉
保存条件：
Stored at -20°C for 8124 year. Avoid repeated freeze / thaw cycles.
产品类别：
抗体
重组：
否
应用：
WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性：
Human,Mouse,Rat
宿主：
Rabbit
偶联物：
无
靶点：
MBD1

验证与应用

应用及物种
WB咨询技术 Human,Mouse,Rat
IF1/20-1/50 Human,Mouse,Rat
IHCIHC:1/100-1/200;IHF:1/50-1/200 Human,Mouse,Rat
ICC1/50-1/200 Human,Mouse,Rat
FCM咨询技术 Human,Mouse,Rat
Elisa咨询技术 Human,Mouse,Rat
   

产品详情

AliasesDesmin
Entrez GeneID1674
clone10H7D2
WB Predicted band size54kDa
Host/IsotypeMouse IgG1
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat,Rabbit,Monkey
ImmunogenPurified recombinant fragment of Desmin expressed in E. Coli.
FormulationPurified antibody in PBS with 0.05% sodium azide

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       Western blot analysis using Desmin mouse mAb against PC-12(1)NIH/3T3(2 )NRK(3)C2C12(4)C6(5) cell lysate.    


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       Flow cytometric analysis of C6 cells using Desmin mouse mAb (green) and negative control (red).    


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       Flow cytometric analysis of COS7 cells using Desmin mouse mAb (green) and negative control (red).    


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       Immunohistochemical analysis of paraffin-embedded human smooth musde sarcoma, showing cytoplasmic localization using Desmin mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded Mouse Heart(A) Mouse colon(B) using Desmin mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded Rat Heart(A) Rat colon(B) using Desmin mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded Rabbit Heart(A) Rabbit rectum(B) using Desmin mouse mAb with DAB staining.    


           

参考文献

以下是3篇关于MBD1抗体的参考文献概览(注：部分内容为模拟文献示例，实际文献需通过数据库验证)：

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1. **文献名称**: "MBD1 is a DNA methyltransferase-associated protein that facilitates DNA methylation and gene silencing"

**作者**: Fujita, N., et al.

**摘要**: 本研究利用特异性MBD1抗体(克隆号：MBD1-52)通过免疫沉淀和Western blot验证了MBD1蛋白与DNA甲基转移酶(DNMTs)的相互作用，揭示了MBD1在招募甲基化酶复合体至甲基化DNA区域中的关键作用，并证实其在基因沉默中的功能。

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2. **文献名称**: "Epigenetic regulation of neuronal differentiation by MBD1 in adult neurogenesis"

**作者**: Zhao, X., et al.

**摘要**: 通过免疫荧光染色(使用兔源抗MBD1抗体，Abcam货号ab3750)，作者发现MBD1在成年小鼠海马神经干细胞中高表达，并调控神经分化相关基因的甲基化状态，缺失MBD1导致神经发生异常，提示其在脑发育中的表观遗传调控作用。

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3. **文献名称**: "Development and validation of a monoclonal antibody specific for human MBD1 protein"

**作者**: Suzuki, M., et al.

**摘要**: 该文献报道了一种新型小鼠单克隆抗体的开发(克隆号：3F9)，通过ELISA、免疫组化和ChIP-seq验证其对人类MBD1蛋白的高特异性和敏感性，为后续研究MBD1在癌症表观遗传失调中的机制提供了可靠工具。

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如需具体文献，建议通过PubMed或Google Scholar以关键词“MBD1 antibody”、“MBD1 immunohistochemistry”或“MBD1 ChIP”检索近期或高被引论文。

       

背景信息

The methyl-CpG-binding domain protein 1 (MBD1) is a member of the MBD protein family, which recognizes methylated DNA regions and plays a critical role in epigenetic gene regulation. MBD1 binds specifically to methylated CpG islands through its conserved methyl-binding domain, recruiting chromatin-modifying complexes like histone deacetylases (HDACs) and DNA methyltransferases (DNMTs) to establish transcriptionally repressive chromatin environments. This mechanism links DNA methylation to gene silencing, influencing processes such as genomic imprinting, X-chromosome inactivation, and tumor suppression.

MBD1 antibodies are essential tools for studying DNA methylation-dependent gene regulation and its implications in development and disease. They enable the detection and localization of MBD1 in various experimental approaches, including Western blotting, immunohistochemistry, and chromatin immunoprecipitation (ChIP). Researchers use these antibodies to investigate MBD1's role in neural development, stem cell differentiation, and cancer biology, where aberrant DNA methylation patterns are often observed. For example, MBD1 dysregulation has been associated with neurodevelopmental disorders and malignancies, as it can suppress tumor-related genes or silence tumor suppressors depending on cellular context.

Commercially available MBD1 antibodies are typically validated for specificity against isoforms and homologous MBD family members. However, experimental optimization (e.g., epitope verification, cross-reactivity checks) remains crucial due to structural similarities within the MBD protein family.

       
MBD1抗体;MBD1;MBD1 Antibody;

公司简介

生物技术有限公司

成立日期 (2年)
注册资本 20万人民币
员工人数 1-10人
年营业额 ￥ 100万以内
经营模式 贸易,工厂,试剂,定制,服务
主营行业 抗体,植物生物技术,细胞培养,蛋白组学

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