DNAPolymerasebeta抗体;DNAPolymerasebeta Antibody—艾普蒂

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     Western blot analysis of DNA Polymerase beta expression in A431 cell lysate.    

  
  

DNA polymerase beta (Pol β) is a key enzyme in the base excision repair (BER) pathway, responsible for repairing small DNA lesions caused by oxidation, alkylation, or deamination. As the primary polymerase for gap-filling during BER, Pol β ensures genomic stability by accurately synthesizing short DNA patches. Antibodies targeting Pol β are essential tools for studying its expression, localization, and functional roles in DNA repair mechanisms.

These antibodies are widely used in research to detect Pol β protein levels in various biological samples via techniques like Western blotting, immunohistochemistry (IHC), and immunocytochemistry (ICC). They help investigate Pol β's involvement in cellular responses to DNA damage, its interaction with other repair proteins (e.g., XRCC1), and its potential role in cancer progression. Altered Pol β expression has been linked to tumorigenesis, chemotherapy resistance, and neurodegenerative disorders, making its antibodies valuable for both basic research and translational studies.

Most Pol β antibodies are raised in rabbits or mice using recombinant protein fragments or synthetic peptides as immunogens. Monoclonal antibodies offer high specificity, while polyclonal versions may detect multiple epitopes. Commercial antibodies are typically validated for cross-reactivity and performance across species (human, mouse, rat). Researchers also utilize Pol β antibodies to explore its post-translational modifications (e.g., phosphorylation) and regulatory mechanisms under genotoxic stress. As a biomarker, Pol β expression patterns may inform therapeutic strategies targeting DNA repair pathways in cancer treatment.