HistoneH3(monomethylK79)抗体;HistoneH3(monomethylK79) Antibody—艾普蒂 新品

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     Western blot analysis of Histone H3 (mono methyl K79) expression in HeLa cell lysate.    

  
  

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

The Histone H3 monomethylK79 (H3K79me1) antibody is a key tool for studying epigenetic regulation linked to gene transcription and chromatin dynamics. Histone H3 lysine 79 (H3K79) is a conserved residue within the globular core of the histone H3. and its methylation is catalyzed by the methyltransferase DOT1L. Unlike other histone modifications, H3K79 methylation occurs on the histone core rather than the N-terminal tail. Monomethylation at K79 (H3K79me1) is associated with active transcription, DNA repair, and cell cycle regulation. It often marks euchromatic regions and plays a role in maintaining genomic stability.

This antibody specifically detects the monomethylated form of H3K79. distinguishing it from unmethylated, di-, or trimethylated states. It is widely used in chromatin immunoprecipitation (ChIP), immunofluorescence (IF), and Western blotting (WB) to investigate spatial and temporal patterns of H3K79me1 in various biological contexts. Studies employing this antibody have revealed its involvement in developmental processes, cancer (e.g., leukemias with MLL rearrangements), and responses to DNA damage.

Validation of H3K79me1 antibodies typically includes knockout cell lines or enzymatic demethylation controls to ensure specificity. Its application has advanced understanding of DOT1L-mediated epigenetic mechanisms and their therapeutic targeting in diseases.