HistoneH2B(acetylK16)抗体;HistoneH2B(acetylK16) Antibody—艾普蒂 新品

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     Western blot analysis of Histone H2B (acetyl K16) expression in HeLa treated with Trichostatin A cell lysate.    

  
  

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     All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.    

  
  

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     All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.    

  
  

The histone H2B (acetylK16) antibody is a specialized tool used to study post-translational modifications of histone H2B, specifically acetylation at lysine 16 (K16). Histones, including H2B, are core components of nucleosomes, playing critical roles in chromatin structure and gene regulation. Acetylation of histones, such as at H2B-K16. is a dynamic epigenetic modification associated with transcriptional activation. This modification neutralizes the positive charge of lysine residues, reducing their interaction with negatively charged DNA and promoting a more open chromatin state, thereby facilitating access for transcriptional machinery.

The H2B-K16 acetylation site is linked to diverse cellular processes, including DNA repair, replication, and chromatin remodeling. Studies suggest its involvement in regulating gene expression during development and stress responses. Antibodies targeting acetylated H2B-K16 enable researchers to investigate these processes through techniques like chromatin immunoprecipitation (ChIP), immunofluorescence, and Western blotting. Specificity validation for such antibodies typically includes peptide competition assays and comparisons with acetylation-deficient mutants to ensure minimal cross-reactivity with other histone modifications.

Research using this antibody has contributed to understanding epigenetic mechanisms in diseases such as cancer, where aberrant histone acetylation patterns are often observed. It remains a vital reagent for exploring the functional significance of site-specific histone modifications in chromatin dynamics and cellular physiology.