Versican抗体;Versican Antibody—艾普蒂 新品

+ +

•  

   

     Western blot analysis of Versican expression in Mouse heart cell lysate.    

  
  

•  

   

     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

Versican, a large extracellular matrix proteoglycan, plays critical roles in tissue morphogenesis, inflammation, and cancer progression. It belongs to the aggrecan/versican/neurocan family and consists of an N-terminal G1 domain, a central chondroitin sulfate (CS)-attachment region, and a C-terminal G3 domain. Alternative splicing generates four isoforms (V0. V1. V2. V3) with varying CS chain numbers, influencing cell adhesion, proliferation, and migration through interactions with hyaluronan, collagen, and cell surface receptors like CD44.

Versican antibodies are essential tools for studying its expression, localization, and functions. Commonly used clones (e.g., 12C5. D8E5) target specific domains: anti-G1 antibodies (e.g., 12C5) detect full-length versican and its processing fragments, while anti-G3 antibodies (e.g., D8E5) help assess proteolytic cleavage or isoform-specific roles. These antibodies are widely applied in techniques like Western blotting, immunohistochemistry, and immunofluorescence to analyze versican distribution in tissues, particularly in contexts like embryonic development, wound healing, and tumor microenvironments.

Dysregulated versican expression correlates with diseases including atherosclerosis, fibrosis, and metastatic cancers. Antibodies enable researchers to explore versican’s dual roles—either promoting or inhibiting tumor growth depending on cellular context—and its potential as a therapeutic target. Validation using knockout models or siRNA knockdown ensures specificity, as cross-reactivity with other proteoglycans remains a concern. Overall, versican antibodies provide critical insights into extracellular matrix dynamics and disease mechanisms.