Y14抗体;Y14 Antibody—艾普蒂 新品

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     Western blot analysis of Y14 expression in HepG2 cell lysate.    

  
  

The Y14 antibody, also known as anti-Y14 or MAGOH antibody, is a widely used tool in molecular biology to study RNA metabolism and splicing mechanisms. It specifically recognizes the Y14 protein (also called MAGOH), a core component of the exon junction complex (EJC). The EJC is a dynamic multiprotein assembly deposited on spliced mRNAs during pre-mRNA processing, playing critical roles in mRNA surveillance, nonsense-mediated decay (NMD), nuclear export, and translation regulation.

Y14. in association with other EJC components like eIF4A3 and BTZ/MLN51. marks spliced mRNAs and participates in quality control pathways. Research using Y14 antibodies has been pivotal in elucidating EJC dynamics, including its recruitment to mRNA, interactions with transport factors, and involvement in diseases linked to RNA misprocessing. For instance, altered EJC function has been implicated in cancer and neurodevelopmental disorders.

The antibody is commonly employed in techniques such as immunoprecipitation, Western blotting, and immunofluorescence to map EJC localization, study RNA-protein interactions, or assess splicing efficiency. Its specificity makes it valuable for both basic research and clinical studies exploring RNA biology. Recent studies also utilize Y14 antibodies to investigate viral RNA hijacking mechanisms and therapeutic targets in conditions with disrupted RNA homeostasis.