NFIB/NF1B2抗体;NFIB/NF1B2 Antibody—艾普蒂 新品

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     Western blot analysis of NFIB / NF1B2 expression in NIH/3T3 cell lysate.    

  
  

The NFIB/NF1B2 antibody targets the nuclear factor I/B (NFIB), a transcription factor belonging to the Nuclear Factor I (NFI) family. NFIB plays critical roles in regulating gene expression during development and cellular differentiation. It binds to DNA through a conserved N-terminal domain, facilitating transcriptional activation or repression of target genes involved in organogenesis, particularly in the lung, brain, and skeletal systems. NFIB also interacts with chromatin-modifying complexes to influence epigenetic regulation. Dysregulation of NFIB is linked to developmental disorders and cancers, such as adenoid cystic carcinoma (ACC), where chromosomal rearrangements or amplifications of NFIB drive tumor progression.

The NFIB/NF1B2 antibody is widely used in research to study NFIB's expression patterns, subcellular localization, and functional mechanisms. It aids in detecting NFIB in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF), helping to correlate its expression with disease states or developmental stages. Additionally, it is employed in chromatin immunoprecipitation (ChIP) to map NFIB-binding genomic regions. Commercial NFIB antibodies are typically validated for specificity against human, mouse, or rat samples, with isoforms (e.g., NFIB-S, NFIB-L) requiring careful selection based on experimental goals. Understanding NFIB's role through such antibodies contributes to insights into tissue-specific regulatory networks and potential therapeutic targets.