Pumilio1抗体;Pumilio1 Antibody—艾普蒂 新品

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     Western blot analysis of Pumilio 1 expression in HeLa cell lysate.    

  
  

Pumilio1 (PUM1) is a member of the evolutionarily conserved PUF family of RNA-binding proteins, which play critical roles in post-transcriptional gene regulation by binding to specific sequences in the 3' untranslated regions (UTRs) of target mRNAs. This interaction typically represses translation or promotes mRNA degradation, influencing processes such as development, neurogenesis, and stem cell maintenance. PUM1 is particularly notable for its involvement in neuronal function, gametogenesis, and embryogenesis. Dysregulation of PUM1 has been linked to neurological disorders, including spinocerebellar ataxia, and cancer progression.

Antibodies against PUM1 are essential tools for studying its expression, localization, and molecular interactions. These antibodies are commonly generated in rabbits or mice using immunogenic peptides or recombinant protein fragments unique to PUM1. Validation often includes Western blotting to confirm specificity for the ~120 kDa protein, immunohistochemistry to assess tissue distribution (e.g., strong expression in brain and testes), and immunoprecipitation to study RNA-protein complexes. Commercial PUM1 antibodies are widely used in research exploring RNA metabolism, developmental biology, and disease mechanisms. However, users must verify cross-reactivity with paralogs like PUM2 and optimize conditions for specific applications. Reliable PUM1 antibodies have advanced studies on its role in mRNA stability, microRNA-mediated regulation, and its potential as a therapeutic target.