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MAD3抗体;MAD3 Antibody—艾普蒂新品

Rabbit Monoclonal MAD3 Antibody

询价 20μl 起订

50μl 起订

100μl 起订

上海更新日期：2025-05-30

上海切尔齐生物科技有限公司

非会员

联系人：周***

电话：027***

手机：180***

邮箱：nuo***

产品详情:

中文名称：: MAD3抗体

英文名称：: Rabbit Monoclonal MAD3 Antibody

品牌：: 艾普蒂

产地：: 武汉

保存条件：: Stored at -20°C for 8709 year. Avoid repeated freeze / thaw cycles.

产品类别：: 抗体

重组：: 否

应用：: WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA

种属反应性：: Human,Mouse,Rat

宿主：: Rabbit

偶联物：: 无

靶点：: MAD3

验证与应用

应用及物种

WB	咨询技术	Human,Mouse,Rat
IF	咨询技术	Human,Mouse,Rat
IHC	1/100-1/200	Human,Mouse,Rat
ICC	技术咨询	Human,Mouse,Rat
FCM	1/20-1/100	Human,Mouse,Rat
Elisa	咨询技术	Human,Mouse,Rat

产品详情

Aliases	bHLHc13; mxd3; Myx;;MAD3
WB Predicted band size	Calculated MW: 23 kDa ; Observed MW: 28 kDa
Host/Isotype	Rabbit IgG
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse,Rat
Immunogen	A synthesized peptide derived from human MAD3
Formulation	Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol.

+ +

Western blot analysis of MAD3 expression in HeLa cell lysate.

参考文献

以下是关于MAD3抗体的3篇参考文献，按研究内容和抗体应用整理：

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1. **文献名称**: *The Mad2 spindle checkpoint protein has two distinct natively folded states*

**作者**: Fang, G., Yu, H., & Kirschner, M.W.

**摘要**: 该研究通过免疫沉淀和Western blot分析，揭示了MAD2蛋白在纺锤体检查点中的构象变化。研究使用MAD3抗体验证了MAD2与MAD3(BUBR1)的相互作用，证明两者共同调控APC/C的活性，确保染色体正确分离。

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2. **文献名称**: *Checkpoint inhibition of the APC/C in HeLa cells is mediated by a complex of BUBR1. BUB3. CDC20. and MAD2*

**作者**: Sudakin, V., Chan, G.K., & Yen, T.J.

**摘要**: 通过免疫共沉淀和抗体阻断实验，该研究鉴定了由MAD3(BUBR1)、BUB3、CDC20和MAD2组成的纺锤体检查点复合物。MAD3抗体被用于特异性识别复合物组分，并证实其在延缓APC/C活化中的关键作用。

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3. **文献名称**: *BUBR1 deficiency results in accelerated mammary tumorigenesis via impaired mitotic checkpoint*

**作者**: Taylor, S.S., Hussein, D., & Wang, Y.

**摘要**: 本研究利用MAD3/BUBR1特异性抗体，通过免疫组织化学和Western blot分析，发现BUBR1缺失导致小鼠乳腺肿瘤易感性增加。结果强调了MAD3在维持有丝分裂检查点及基因组稳定性中的功能。

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**备注**：MAD3常被称为BUBR1(BUB1-related kinase)，上述文献中使用的抗体多针对其蛋白表达及相互作用研究，涉及技术包括Western blot、免疫沉淀等。如需更近期研究，建议检索PubMed等数据库以获取最新进展。

背景信息

MAD3 (Mitotic Arrest Deficient 3) antibody is primarily associated with the detection of BubR1. a critical protein involved in the spindle assembly checkpoint (SAC) during mitosis. BubR1. encoded by the *BUB1B* gene, ensures accurate chromosome segregation by delaying anaphase until all chromosomes are properly attached to spindle microtubules. Dysfunction in BubR1 is linked to chromosomal instability, a hallmark of cancer, and has been implicated in aging-related disorders.

The MAD3 antibody recognizes BubR1. enabling researchers to study its expression, localization, and interactions in cell cycle regulation. It is widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate SAC mechanisms, mitotic errors, and cellular responses to anti-mitotic drugs. Studies using MAD3 antibodies have revealed BubR1's role in aging, as reduced levels correlate with age-related phenotypes in model organisms. Additionally, its involvement in cancer progression makes it a potential biomarker or therapeutic target.

Originally identified in yeast, the term "MAD3" is sometimes used interchangeably with BubR1 in vertebrates, though nomenclature varies. Researchers employ MAD3 antibodies to explore molecular pathways governing genomic stability, providing insights into diseases like aneuploidy-driven cancers and developmental disorders. Its utility spans basic cell biology, translational oncology, and aging research.

MAD3抗体;MAD3;MAD3 Antibody;

公司简介

生物技术有限公司

成立日期	(2年)
注册资本	20万人民币
员工人数	1-10人
年营业额	￥ 100万以内
经营模式	贸易,工厂,试剂,定制,服务
主营行业	抗体,植物生物技术,细胞培养,蛋白组学