食蟹猴PSMA / FOLH1蛋白, His Tag

Cynomolgus PSMA / FOLH1 Protein, His Tag (active enzyme, MALS verified)

分子别名（Synonym）

FOLH1,PSMA,GIG27,FOLH,NAALAD1,PSM,NAALADase I,GCPII,FGCP

表达区间及表达系统（Source）

Cynomolgus PSMA, His Tag (PSA-C5247) is expressed from human 293 cells (HEK293). It contains AA Lys 44 - Ala 750 (Accession # A0A2K5VNZ0-1).

Predicted N-terminus: His

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 81.4 kDa. The protein migrates as 100-115 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method / rFC method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Supplied as 0.2 μm filtered solution in MES and NaCl with trehalose as protectant.

Contact us for customized product form or formulation.

运输（Shipping）

This product is supplied and shipped with dry ice, please inquire the shipping cost.

存储（Storage）

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

The product MUST be stored at -70°C or lower upon receipt;

-70°C for 3 months under sterile conditions.

背景介绍

前列腺特异性膜抗原（PSMA）又称叶酸水解酶1（FOLH1）、谷氨酸羧肽酶2（GCP2）、N-乙酰化α连接酸性二肽酶I（NAALAD1），属于肽酶M28家族及M28B亚家族。FOLH1/PSMA在pH值大于6.5的环境中保持稳定，是一种II型跨膜锌金属肽酶，在神经系统、前列腺、肾脏和小肠中表达量最高。FOLH1/GCP-2以同源二聚体形式存在，每个亚基可结合2个锌离子，是维持NAALADase活性所必需的。

PSMA的催化活性主要体现为催化未取代的C端谷氨酰基团水解，其典型底物为Ac-Asp-Glu或叶酰聚-γ-谷氨酸盐。FOLH1/GCP-2/PSMA同时具有叶酸水解酶和N-乙酰化α连接酸性二肽酶（NAALADase）活性，并对三-α-谷氨酸肽具有特异性偏好。GCP-2/PSMA参与前列腺肿瘤进展过程，同时表现出二肽基肽酶IV型活性，在体外实验中可切割Gly-Pro-AMC底物。