Human Integrin alpha 4 beta 7 (ITGA4&ITGB7) Heterodimer Protein, His Tag&Tag Free (MALS & SPR verified)
分子别名（Synonym）
Integrin alpha 4 beta 7,ITGA4&ITGB7
表达区间及表达系统（Source）
Human ITGA4&ITGB7 Heterodimer Protein, His Tag&Tag Free (IT7-H52W4) is expressed from human 293 cells (HEK293). It contains AA Tyr 34 - Thr 977 (ITGA4) & Glu 20 - His 723 (ITGB7) (Accession # P13612-1 (ITGA4) & P26010-1 (ITGB7)).
Predicted N-terminus: Tyr 34 (ITGA4) & Glu 20 (ITGB7)
蛋白结构（Molecular Characterization）
Human ITGA4&ITGB7 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGA4 and ITGB7, has a calculated MW of 111.4 kDa (ITGA4) & 83.7 kDa (ITGB7). Subunit ITGA4 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB7 contains no tag but a basic tail at the C-terminus. The protein migrates as 130-150 kDa (ITGA4) and 65 kDa, 70 kDa, 100-120 kDa (ITGB7) when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method / rFC method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
背景介绍
整合素α4β7是一种黏附受体，属于整合素家族，与α4β1（VLA4）及E-钙黏蛋白受体αEβ7共享亚基。该受体对淋巴细胞归巢至肠道黏膜至关重要，其表达在派尔集合淋巴结（Peyer’s patches）或肠系膜淋巴结的T细胞活化过程中被诱导产生，且依赖于局部树突细胞与基质细胞分泌的视黄酸等信号分子。
HIV-1包膜蛋白gp120可与活化状态的整合素α4β7结合，此相互作用可能（但也可能无关）解释HIV-1病毒在肠道相关淋巴组织（GALT）中的富集现象。此外，整合素α4β7还可能参与急性肠道移植物抗宿主病（GVHD）中的淋巴细胞迁移过程。
