Human Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein, His Tag&Tag Free (MALS verified)
分子别名（Synonym）
Integrin alpha V beta 6,ITGAV&ITGB6
表达区间及表达系统（Source）
Human ITGAV&ITGB6 Heterodimer Protein, His Tag&Tag Free (IT6-H52E1) is expressed from human 293 cells (HEK293). It contains AA Phe 31 - Val 992 (ITGAV) & Gly 22 - Asn 707 (ITGB6) (Accession # NP_002201.1 (ITGAV) & AAI21179.1 (ITGB6)).
Predicted N-terminus: Phe 31 (ITGAV) & Gly 22 (ITGB6)
蛋白结构（Molecular Characterization）
Human ITGAV&ITGB6 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGAV and ITGB6, has a calculated MW of 112.9 kDa (ITGAV) & 79.6 kDa (ITGB6). Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB6 contains no tag but a basic tail at the C-terminus. The protein migrates as 85 kDa (ITGB6) and 140 kDa (ITGAV) when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method / rFC method.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
背景介绍
整合素αVβ6是由αV与β6亚基构成的异二聚体，作为纤连蛋白（fibronectin）和细胞黏连蛋白（cytotactin）的受体，可特异性识别配体中的R-G-D序列。该受体通过网格蛋白介导的内吞作用促进癌细胞侵袭，同时作为柯萨奇病毒A9/B1及单纯疱疹病毒-1（HHV-1）的感染受体。此外，其结合TGF-β潜伏相关肽（LAP）后，可激活大分子潜伏复合物中的TGF-β1/β3——该过程需与LTBP-1及纤连蛋白相互作用，并受PAR-1增强调控。
