Human Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer Protein, His Tag&Tag Free
分子别名（Synonym）
Integrin alpha V beta 8, ITGAV&ITGB8
表达区间及表达系统（Source）
Human ITGAV&ITGB8 Heterodimer Protein, His Tag&Tag Free (IT8-H52W4) is expressed from human 293 cells (HEK293). It contains AA Phe 31 - Val 992 (ITGAV) & Glu 43 - Arg 684 (ITGB8) (Accession # NP_002201.1 (ITGAV) & NP_002205.1 (ITGB8)).
Predicted N-terminus: Phe 31 (ITGAV) & Glu 43 (ITGB8)
蛋白结构（Molecular Characterization）
Human ITGAV&ITGB8 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGAV and ITGB8, has a calculated MW of 112.9 kDa (ITGAV) and 76.5 kDa (ITGB8). Subunit ITGAV is fused with polyhistidine tag at the C-terminus and followed by a acidic tail and subunit ITGB8 contains no tag but a basic tail at the C-terminus. The non-reducing (NR) protein migrates as 130-150 kDa (ITGAV) and 80-90 kDa (ITGB8) when calibrated against Star Ribbon Pre-stained Protein Marker respectively due to glycosylation.
内毒素（Endotoxin）
Less than 0.01 EU per μg by the LAL method / rFC method.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM NaCl, pH8.0 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
背景介绍
整合素αVβ8（由ITGAV与ITGB8基因编码）表达于卵黄囊、胎盘、脑血管周围星形胶质细胞、施万细胞、肾小球系膜细胞及肺上皮细胞。与其他αV整合素不同，αVβ8似乎不具备不同的激活状态，其胞内尾部不与细胞骨架连接。该整合素能够结合含RGD基序的配体，包括玻连蛋白、纤维蛋白以及潜伏转化生长因子-β复合体中的潜伏相关肽。αVβ8与潜伏相关肽的高亲和力结合使其能被MT1-MMP蛋白酶切，从而释放活性TGF-β。此机制不同于另一个可激活TGF-β的αV整合素——αVβ6，后者通过非蛋白酶解机制实现TGF-β从潜伏状态的激活。TGF-β激活的下游效应包括调控细胞生长及相关血管生成过程。
