人GPR75蛋白, Flag,His Tag

Human GPR75 Full Length Protein, Flag,His Tag (Detergent)

分子别名（Synonym）

GPRchr2, WI31133

表达区间及表达系统（Source）

Human GPR75 Full Length Protein, Flag,His Tag (GP5-H52D3) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Val 540 (Accession # O95800).

Predicted N-terminus: Asp

蛋白结构（Molecular Characterization）

This protein carries a flag tag at the N-terminus and a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 78.4 kDa.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method / rFC method.

纯度（Purity）

>90% as determined by SDS-PAGE.

制剂（Formulation）

This product is not suitable for cell based experiments due to cytotoxicity of DDM.

DDM and CHS are INDISPENSABLE to keep membrane protein soluble and active, under no circumstances should you remove DDM and CHS.

DDM/CHS buffer (DC-11) is sold separately and not included in protein, and please contact us if you need the buffer.
If glycerol is not compatible with your application, remove glycerol just before the immediate experiment, and NEVER store glycerol-free protein solution.

Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, DDM, CHS, pH7.5 with glycerol as protectant.

Contact us for customized product form or formulation.

运输（Shipping）

This product is supplied and shipped with dry ice, please inquire the shipping cost.

存储（Storage）

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

The product MUST be stored at -70°C or lower upon receipt;

-70°C for 3 months under sterile conditions.

背景介绍

该受体是由趋化因子CCL5/RANTES激活的G蛋白偶联受体。它可能与异源三聚体Gq蛋白偶联，在激活后刺激三磷酸肌醇的产生和钙离子的动员。该受体与CCL5/RANTES协同作用，可能通过激活涉及PI3激酶、Akt激酶和MAP激酶的下游信号通路，在神经元存活中发挥作用。CCL5/RANTES还可能通过激活该受体来调节胰岛细胞的胰岛素分泌。