Cosmetic Application of Sodium Lauroyl Glutamate

Sodium lauroyl glutamate (SLG) is a mild cleansing agent often used in rinse-off skin care products. SLG’s the salt form of the combination of amino acid glutamic acid and lauric acid, a fatty acid commonly sourced from coconut oil. As a surfactant, SLG helps cleanse skin, which is why it’s commonly included in face washes and cleansers. Because of the amino and fatty acids present in SLG, it’s often referred to as a great option for sensitive skin. It imparts a smooth and soft feeling after use. The Cosmetic Ingredient Review Expert Panel reviewed SLG and deemed it safe for use in cosmetics. It can appear in cosmetics and hair care in amounts ranging from 0.003% all the way to 40%. In its raw material state, Sodium lauroyl glutamate appears as a white or pale-yellow powder.

Rapid porcine corneal decellularization through the use of Sodium lauroyl glutamate

As the most common transplant, corneal transplantation is apparently the first choice for the treatment of corneal blindness. Corneal stroma accounts for 90% of the corneal thickness, and several equivalents, such as the polymethacrylates synthetic keratoprosthesis, collagen biosynthetic implants, and cell-based replacements, have been developed for the substitution of diseased stroma. However, these substitutes have been reported to induce serious complications, suture intolerance, or unsatisfactory recovery of visual acuity. Therefore, decellularized xenogenic cornea represents a promising alternative source of human donor. With commendable properties, like mild, strong decontamination, and good compatibility, amino acid-based surfactants such as sodium N-lauroyl glutamate (SLG) and Sodium lauroyl glutamate (SLS) have been widely used in daily supplies like skin care products, shower gels, and detergents.In this present study, we aimed to develop a better decellularization method by using Sodium lauroyl glutamate and supernuclease, in order to overcome the structural and functional impairment of DPCs processed by traditional decellularization methods. For the recommendation of an optimum method, we then tested the key properties of DPCs decellularized by our and another two traditional methods, and examined the postoperative symptoms of rabbit corneas after the implementation of lamellar keratoplasty (LKP) with DPCs.[1]

Here we provide a modified method by using the mild detergent SLG and supernuclease for the decellularization of porcine corneal stroma. Results indicated that our decellularization method presented a higher removal efficiency (within 3 h) of the xenogenic porcine antigens, and protected the DPCs with a normal-like ultrastructural integrity and transparency. Moreover, the better structural and functional advances were presented in the DPCs treated with Sodium lauroyl glutamate and supernuclease compared with porcine corneal stroma decellularized by traditional decellularization methods. The application effects of glycerol on transparency improvement might disguise the internal structural damages of porcine cornea stroma caused by the over-swelling during decellularization process, but a foreseeable postoperative complication is about the transparency decline of the reconstructed corneas because of the removal of glycerol. Furthermore, good postoperative outcomes were showed in rabbits after performing the LKP with porcine corneal stroma decellularized with Sodium lauroyl glutamate and supernuclease, showing an eligible thickness of the reconstructed rabbit corneas, and no significant corneal edema appeared.

In addition, a high mechanical strength is essential for the survival of decellularized corneal grafts, as they need to withstand the stitch tear during the surgery, as well as the membrane stresses caused by intraocular pressure and external forces. Based on the superior characteristics of the presented DPCs, the combined use of detergent Sodium lauroyl glutamate and supernuclease may serve as a promising method for the clinical use of DPCs, and provide a reference method for decellularization in other fields. The combined use of Sodium lauroyl glutamate and supernuclease is a recommendable method for the production of DPCs, which demonstrated a high removal efficiency of xenogenic cells and antigens in porcine corneas, together with the better protective function on ultrastructural integrity and biological characteristics of corneal matrix fibers compared with traditional decellularization methods.

References

[1]Dong M, Zhao L, Wang F, Hu X, Li H, Liu T, Zhou Q, Shi W. Rapid porcine corneal decellularization through the use of sodium N-lauroyl glutamate and supernuclease. J Tissue Eng. 2019 Sep 11;10:2041731419875876. doi: 10.1177/2041731419875876. PMID: 31588337; PMCID: PMC6740050.

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