What is the Nisin by SPPS?

Batch fermentation Batch fermentations were conducted in 1 1 Quickfit-type fermenters at 30°C with the pH maintained at 6.5 by automatically feeding 5 M NaOH. Alternatively, AmberliteIRA 67 was manually added to the fermenters to maintain the pH at 6.5. The fermentations were continually mixed with a magnetic stirrer. Overnight cultures were used as inoculum at 10% v/v. Samples (1 ml) were collected hourly for turbidity and lactate measurements and for nisin assay. The samples for turbidity measurement were appropriately diluted in saline and measured at 620 nm. The samples for nisin assay were adjusted to give a pH of 2 ± 0.5 with a few drops of conc HCl and were stored at 4°C[1].
References [1] P. Yu, W. Kim, N. Dunn. “Lactate removal by anionic-exchange resin improves nisin production by Lactococcuslactis.”Biotechnology Letters 24 1 (2004): 59–64.

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